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KMID : 0903520060490020091
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2006 Volume.49 No. 2 p.91 ~ p.96
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Antioxidant Activities and Biological Properties of Phellinus linteus Extracts according to Different Extraction Methods
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Kwoen Dae-Jun
Youn Sun-Joo
Cho Jun-Gu
Choi Ung-Kyu
Kang Sun-Chul
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Abstract
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Antioxidant activities and biological properties such as antimutagenic and cytotoxic effect of Phellinus linteus extracts from different extraction conditions were measured against Salmonella typhimurium and human cancer cell lines. DPPH free radical scavenging activities of the extracts were higher in the solutions extracted with ethanol (17.14) and ethanol after water (17.79), respectively. In the Ames test, ethanol extract of P. linteus alone did not exhibit any mutagenicity but showed substantial inhibitory effect against mutations induced by N-methyl-N\’-nitro-N-nitrosoguanidine (MNNG), 4-nitroquinoline-1-oxide (4NQO), and benzo (α) pyrene (B(α)P). The extracts of ethanol and ethanol after water of P. linteus (200 μg/plate) had the highest inhibitory effect of 61.5 and 60.9%, respectively, on the mutagenesis on S. typhimurium TA98 strain induced by B(α)P. Extracted solutions of ethanol and ethanol after water of P. linteus showed high antimutagenic effect against MNNG, 4NQO, Trp-P-1 and B(α)P, causing mutations in S. typhimurium TA100 strain. The anticancer effects of P. linteus extracts were investigated against human fibrosarcoma HT-29 and human hepatocellular carcinoma HepG2. The treatment of 0.5 mg/ml of ethanol, ethanol after water and water extracts of P. linteus had the highest cytotoxicity of 59, 57, 54%, respectively against HT-27 cell line, whereas low cytotoxicity effects were observed against HepG2 cell line in the range of 10~30%. The ethanol and water extracts of P. linteus also showed the nitrate scavenging ability at different pHs. The ethanol extract showed higher nitrate-scavenging ability compared to water extract of P. linteus.
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KEYWORD
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Phellinus linteus, DPPH, Ames test, antimutagenic effect, cytotoxicity
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